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Please use this identifier to cite or link to this item: http://hdl.handle.net/10077/3795

Title: Coffee species and varietal identification
Authors: Tornincasa, Patrizia
Furlan, Michela
Pallavicini, Alberto
Graziosi, Giorgio
Keywords: adulteration
Coffea arabica
Coffea canephora
microsatellites
multiplex PCR
real time PCR
roasted coffee
traceability
Issue Date: 2010
Publisher: EUT Edizioni Università di Trieste
Citation: Patrizia Tornincasa [et al.], Coffee species and varietal identification, in Pier Luigi Nimis and Régine Vignes Lebbe (eds.): “Tools for Identifying Biodiversity: Progress and Problems. Proceedings of the International Congress, Paris, September 20-22, 2010”, Trieste, EUT Edizioni Università di Trieste, 2010, pp. 307-313.
Abstract: There are serious economical reasons to pretend warranties in coffee species and varieties authenticity. Arabica adulteration with Robusta coffees, intentional or not, is carried out at different steps of the coffee chain, from plantation to beverage. We present a method based on a real-time PCR technique to perform: a) a qualitative analysis to evaluate the presence/ absence of a species in a sample; b) a quantitative analysis to amplify Robusta samples only, making possible the detection of less than 5% of Robusta in a mixture. Arabica cultivars sold as specialty coffees can be identified and certified by fingerprinting using SSR markers.
URI: http://hdl.handle.net/10077/3795
ISBN: 978-88-8303-295-0
Appears in Collections:Tools for Identifying Biodiversity: Progress and Problems

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