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Please use this identifier to cite or link to this item: http://hdl.handle.net/10077/3800

Title: DNA Barcoding of Philippine plants
Authors: Agoo, Esperanza Maribel G.
Keywords: barcoding
Philippines
DNA
identification
flora
Issue Date: 2010
Publisher: EUT Edizioni Università di Trieste
Citation: Esperanza Maribel G. Agoo, DNA Barcoding of Philippine plants, in Pier Luigi Nimis and Régine Vignes Lebbe (eds.): “Tools for Identifying Biodiversity: Progress and Problems. Proceedings of the International Congress, Paris, September 20-22, 2010”, Trieste, EUT Edizioni Università di Trieste, 2010, p. 341.
Abstract: DNA barcoding is a technique that uses DNA sequence data for species-level identification, for analyzing phylogenies and interspecific variation and in population genetic studies. The DNA barcodes determined by the Consortium of the Barcode of Life (CBOL) to be the most effective in achieving these goals in plants are the plastid genes namely trnH-psbA, rbcL, matK, accD, rpoB, rpoc1, and trnL(UAA)-trnF(GAA). The goal of this barcoding project is to test the genes, trnH-psbA, rbcL, and matK, in identification and describing variation in some Philippine noteworthy indigenous plant groups such as orchids, gingers, aroids, cinnamons, and cycads. DNA are extracted and processed using standard protocol set by the CBOL. The DNA are then kept in a cold storage facility in the DLSUCENSER laboratory. Voucher specimens are also collected and are now deposited in the DLSU-Manila Herbarium. Results of this study show that these candidate barcodes can successfully discriminate species including probable novelties of cycads, aroids, and cinnamons. Quantitative analysis also suggest that rbcL and trnHpsbA are very variable genes and can reflect greater interspecific variation thus are the more useful barcodes in these plant groups.
URI: http://hdl.handle.net/10077/3800
ISBN: 978-88-8303-295-0
Appears in Collections:Tools for Identifying Biodiversity: Progress and Problems

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