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Title: Identification of novel protein scaffolds for small molecules binding and for catalysis
Other Titles: Identificazione di nuovi scaffold proteici per il legame di piccole molecole e per la catalisi
Authors: Luisi, Immacolata
Supervisor/Tutor: Berti, Federico
Marzari, Roberto
Co-supervisor: Sblattero, Daniele
Issue Date: 29-Mar-2012
Publisher: Universit√† degli studi di Trieste
Abstract: Protein scaffolds are stable structures capable to recognize and bind, in different conditions, small guest molecules. They are proteins with known conformation which can be used and modified for the construction of variants. The goal of this work is the identification of peptide-based artificial receptors or catalysts. To this purpose, we have considered two different protein motifs to generate new scaffolds: a synthetic E/K coiled-coil domain, and one of the binding sites of the natural protein Human serum albumin. In order to generate stable peptide hosts, we developed peptide libraries to be selected for both properties, adopting two different approaches: RANDOM mutagenesis and SITE-DIRECTED mutagenesis.
PhD cycle: XXIV Ciclo
Description: 2010/2011
Keywords: Protein scaffolds
EK coiled-coil
Human Serum Albumin
Small molecules
Binding and catalysis
Main language of document: en
Type: Tesi di dottorato
Doctoral Thesis
Scientific-educational field: BIO/06 ANATOMIA COMPARATA E CITOLOGIA
NBN: urn:nbn:it:units-9175
Appears in Collections:Scienze biologiche

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