Publication: Antioxidative blood status assessment by the free bilirubin assay
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Date
2014
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Publisher
EUT Edizioni Università di Trieste
Abstract
Bilirubin is an endogenous antioxidant in the human blood. While measurement of a total serum
bilirubin, comprising of protein-bound (indirect bilirubin) and bilirubin glucuronide (direct bilirubin), is a
routine clinical test, direct determination of free bilirubin represents an analytical challenge. Free bilirubin,
which is the bioactive form, accounts for a minor fraction (<100 nM). Here we show the direct analysis of
free bilirubin in human and animal blood serum samples using a state-of-the-art system comprised of newly
developed high-performance liquid chromatography (HPLC) on reversed-phase (RP) C18 support coupled
with thermal lens spectrometric detection (TLS), based on excitation wavelength of 457.9 nm using argon
laser. The method excels in ultra-high sensitivity with limit of detection (LOD) and limit of quantitation (LOQ) of
90 pM and 250 pM, respectively. As a result, we present here the direct assay of free bilirubin in serum (around
10 nM), which can be employed as an assessment of antioxidative blood status.
Description
Keywords
TRANS2CARE, antioxidant, bilirubin, blood, HPLC, DAD, TLS
Citation
Mitja Martelanc, Lovro Žiberna, Mladen Franko, Sabina Passamonti, Antioxidative blood status assessment by the free bilirubin assay, in S. Passamonti, S. Gustincich, T. Lah Turnšek, B. Peterlin, R. Pišot, P. Storici (Eds.), Cross-border Italy-Slovenia biomedical research: are we ready for horizon 2020? Conference proceedings with an analysis of innovation management and knowledge transfer potential for a smart specialization strategy. Trieste, EUT Edizioni Università di Trieste, 2014, pp. 191-195