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Real-time PCR applications in clinical research and diagnostics

Rak, Mitja
•
Barlič–Maganja, Darja
•
Hmeljak, Julija
•
Todorović, Vesna
•
Čemažar, Maja
•
Cor, Andrej
2014
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ISBN
978-88-8303-512-8
http://hdl.handle.net/10077/10042
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Abstract
Polymerase chain reaction (PCR) has enabled enormous progress in the field of molecular biology in the last twenty-five years. It became popular due to its high sensitivity and specificity and was introduced to numerous scientific fields. Today many different variants of PCR exist, with real-time PCR being the most common. This method is being successfully used at our institution for a variety of different applications, among which gene expression analyzes and development of protocols for clinical diagnostics currently dominate. But the extreme flexibility and customizability of real-time PCR, coupled with our team’s expertise, ensures efficient application of this technique to various new research projects.
Subjects
  • real-time PCR

  • SYBR Green

  • TaqMan

  • quantification

  • gene expression

Publisher
EUT Edizioni Università di Trieste
Source
Mitja Rak, Darja Barlič–Maganja, Julija Hmeljak, Vesna Todorović, Maja Čemažar, Andrej Cor, Real-time PCR applications in clinical research and diagnostics, in Sabina Passamonti (ed.), The Partners and the Objectives of Trans2Care, an Italy-Slovenia cross-border network of science and healthcare institutions, EUT - Edizioni Università di Trieste, 2014, pp. 129-132
Languages
en
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