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Title: Real-time PCR applications in clinical research and diagnostics
Authors: Rak, Mitja
Barlič–Maganja, Darja
Hmeljak, Julija
Todorović, Vesna
Čemažar, Maja
Cor, Andrej
Keywords: real-time PCRSYBR GreenTaqManquantificationgene expression
Issue Date: 2014
Publisher: EUT Edizioni Università di Trieste
Source: Mitja Rak, Darja Barlič–Maganja, Julija Hmeljak, Vesna Todorović, Maja Čemažar, Andrej Cor, Real-time PCR applications in clinical research and diagnostics, in Sabina Passamonti (ed.), The Partners and the Objectives of Trans2Care, an Italy-Slovenia cross-border network of science and healthcare institutions, EUT - Edizioni Università di Trieste, 2014, pp. 129-132
Polymerase chain reaction (PCR) has enabled enormous progress in the field of molecular biology in the last twenty-five years. It became popular due to its high sensitivity and specificity and was introduced to numerous scientific fields. Today many different variants of PCR exist, with real-time PCR being the most common. This method is being successfully used at our institution for a variety of different applications, among which gene expression analyzes and development of protocols for clinical diagnostics currently dominate. But the extreme flexibility and customizability of real-time PCR, coupled with our team’s expertise, ensures efficient application of this technique to various new research projects.
Type: Book Chapter
ISBN: 978-88-8303-512-8
Appears in Collections:Trans2Care, 2012. The Partnership and the Objectives of Trans2Care, an Italy-Slovenia cross-border network of science

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