Please use this identifier to cite or link to this item: http://hdl.handle.net/10077/10297
Title: The human recq1 helicase is highly expressed in glioblastoma and plays an important role in tumor cell proliferation
Authors: Faoro, Valentina
et al.
Keywords: GLIOMA projectglioblastomacancer stem cellsRECQ1 helicasegenome stability
Issue Date: 2014
Publisher: EUT Edizioni Università di Trieste
Source: Valentina Faoro, Sasa Kenig, Paola Storici, Alessandro Vindigni, The human recq1 helicase is highly expressed in glioblastoma and plays an important role in tumor cell proliferation, in S. Passamonti, S. Gustincich, T. Lah Turnšek, B. Peterlin, R. Pišot, P. Storici (Eds.), Cross-border Italy-Slovenia biomedical research: are we ready for horizon 2020? Conference proceedings with an analysis of innovation management and knowledge transfer potential for a smart specialization strategy. Trieste, EUT Edizioni Università di Trieste, 2014, pp. 256-260
Abstract: 
RecQ helicases play an essential role in the maintenance of genome stability. In humans, loss
of RecQ helicase function is linked to predisposition to cancer and premature ageing. Distinct molecular
functions for the five human RecQ helicases, and RECQ1 in particular, in replication stress and cancer remain
to be defined. Within the GLIOMA project we are studying the expression and the role of RECQ1 in replication
stress response and repair in glioblastoma and Glioma Stem Cells (GSC). Different papers show that GSCs play
an important role in the initiation, aggressiveness and recurrence of glioblastoma. Here, we show that RECQ1
is highly expressed in various types of solid tumours, but only in the case of glioblastoma, the expression
of RECQ1 is significantly increased in tumoural tissue relative to the surrounding perilesional. We also show
that the depletion of RECQ1 by RNAi results in a significant reduction of cellular proliferation, perturbation of
S-phase progression, and spontaneous γ-H2AX foci formation in T98G and U87 glioblastoma cells. Moreover,
RECQ1 depleted cells are hypersensitive to hydroxyurea or temozolomide treatment. Collectively, these results
indicate that RECQ1 has an important role in the maintenance of genome integrity and might represent a
new suitable target for anti cancer therapies aimed to arrest cell proliferation in glioma. In the framework of
GLIOMA project we are also working to establish a zebrafish cancer model to characterize the role of RECQ1 in
glioblastoma formation and progression in vivo.
Type: Article
URI: http://hdl.handle.net/10077/10297
ISBN: 978-88-8303-572-2
eISBN: 978-88-8303-573-9
Appears in Collections:Trans2Care, 2014. Cross-Border Italy-Slovenia Biomedical Research. Are we ready for Horizon 2020?

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