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Title: Multi & fast: the secret for a protein production facility@elettra
Authors: Giabbai, Barbara
et al.
Keywords: protocols were optimized to obtain correctly folded, functional and stable proteins for structural studies
Issue Date: 2014
Publisher: EUT Edizioni Università di Trieste
Source: Barbara Giabbai, Marta Stefania Semrau, Francesco Bisiak, Jure Gams, Paola Storici, Multi & fast: the secret for a protein production facility@elettra, in S. Passamonti, S. Gustincich, T. Lah Turnšek, B. Peterlin, R. Pišot, P. Storici (Eds.), Cross-border Italy-Slovenia biomedical research: are we ready for horizon 2020? Conference proceedings with an analysis of innovation management and knowledge transfer potential for a smart specialization strategy. Trieste, EUT Edizioni Università di Trieste, 2014, pp. 288-293
Expression, purification and structure determination of recombinant proteins is a fundamental
requirement for a variety of biological applications, from exploratory research to pharmaceutical drug discovery.
Exploiting the expertise and the strategic location of the laboratories of Elettra Synchrotron of Trieste, we
have invested to create a dedicated center for protein production and characterization in the newly renovated
structural biology lab of Elettra. The PROTEO project aims to offer a specialized support to research groups that
need to obtain pure recombinant proteins for functional or structural studies. The core protein production unit
is equipped to express/purify proteins with high efficiency in prokaryotic and eukaryotic systems and is setting
up HT protocols. Currently, ten different projects are running in the lab. For each project, constructs design
followed by fast multi-cloning approach has been performed leading to the combined evaluation of a number
of different expression data: most adequate expression systems were chosen and the purification protocols
were optimized to obtain correctly folded, functional and stable proteins for structural studies.
In this paper we present the organization of the PROTEO protein production platform and a number of studies
Type: Book Chapter
ISBN: 978-88-8303-572-2
eISBN: 978-88-8303-573-9
Appears in Collections:Trans2Care, 2014. Cross-Border Italy-Slovenia Biomedical Research. Are we ready for Horizon 2020?

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