Please use this identifier to cite or link to this item:
|Title:||Molecular mechanisms of pancreas development and insulin regulation in beta cells.||Authors:||Paroni, Federico||Supervisore/Tutore:||Paoletti, Sergio||Cosupervisore:||Bonner Weir, Susan||Issue Date:||1-Apr-2008||Publisher:||Università degli studi di Trieste||Abstract:||
Type 1 or “juvenile” Diabetes is an autoimmune disease in which the insulin secreting beta-cells, essential for glucose homeostasis, are destroyed by a target immune attack. To date, patients with type 1 Diabetes can rely only on exogenous insulin injection to control the glucose levels, but, unfortunately, the related chronic and devastating complications cannot be prevented. Identification of beta-cell-specific genes playing critical roles during the development will help to create new sources of beta-cells from stem/progenitor cells. Interestingly, endocrine cell fate appears to be governed by the “peaked” expression of the transcription factor Ngn3. However, Ngn3’s targets are still not well identified. As for pancreas development also insulin gene regulation lacks important information. Therefore the aims of this Thesis work have been to test an set-up an efficient system for the transient expression of Ngn3, mimicking its physiological behavior; identify the major factors that lie directly downstream the Ngn3 expression and characterize a novel beta-cell specific insulin gene regulator. As result from this work, an innovative system mimicking pancreatic differentiation and Ngn3 pulsed expression has been developed. Furthermore, a novel Ngn3’s downstream target, the zinc-finger protein “OVO_like 1, has been identified opening a new, and interesting, scenario of Ngn3 gene’s regulation. Last, but not least, the beta-cell-insulin-gene regulator A2.2 has been characterized and a reproducible purification system has been developed.
|Keywords:||beta-cell, pancreas, insulin, development, neurgenin 3, gene regulation||Language:||en||Type:||Doctoral Thesis||Settore scientifico-disciplinare:||BIO/12||NBN:||urn:nbn:it:units-7203|
|Appears in Collections:||Scienze biologiche|
Show full item record
Files in This Item:
|Thesis FP.pdf||PhD Thesis Federico Paroni||6.9 MB||Adobe PDF|
checked on Jun 21, 2019
checked on Jun 21, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.