Please use this identifier to cite or link to this item:
Title: Neural stem cells and their interaction with the brain environment
Authors: Cusulin, Carlo
Keywords: TrapiantoFusioneCellule staminali neuralimicrogliaModelli animali
Issue Date: 16-Mar-2010
Publisher: Università degli studi di Trieste
Abstract: The NS culture system is an innovative yet not fully characterized method of culturing neural stem cells (NSCs). Previous reports have described the possibility of isolation of a virtually pure NSC culture from embryonic, fetal or adult NSCs (Conti et al., 2005; Pollard et al., 2006; Sun et al., 2008). These cells, grown in adhesion, are called NS cells and show radial glial characteristics. The present thesis aims to characterize the in vitro and in vivo behaviour of human and mouse NS cells. In the first study, we describe the isolation of a new strain of human striatal NS cells. They show unique features in vitro, and can be efficiently differentiated into neurons. After transplantation in newborn rats, they survive, migrate and differentiate in the host brain. In the second part of the thesis, we show that mouse ES-derived NS cells fuse with cortical pyramidal neurons after transplantation in mice or rats. This process is mediated by microglia, which first fuse with the NS cells and then with the neurons. This NS-microglia-neuron fusion has not been previously described, although it occurs both in vivo and in vitro. In summary, we report here previously undescribed features of the NS cells; our results are relevant to better understanding of NSCs in general and their behaviour after transplantation in the brain.
Description: 2008/2009
NBN: urn:nbn:it:units-8835
Appears in Collections:Scienze biologiche

Files in This Item:
File Description SizeFormat 
Thesis Carlo Cusulin.pdfThesis39.27 MBAdobe PDFView/Open
Show full item record

CORE Recommender

Page view(s)

checked on Mar 17, 2018


checked on Mar 17, 2018

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.