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Title: Coffee species and varietal identification
Authors: Tornincasa, Patrizia
Furlan, Michela
Pallavicini, Alberto
Graziosi, Giorgio
Keywords: adulterationCoffea arabicaCoffea canephoramicrosatellitesmultiplex PCRreal time PCRroasted coffeetraceability
Issue Date: 2010
Publisher: EUT Edizioni Università di Trieste
Source: Patrizia Tornincasa [et al.], Coffee species and varietal identification, in Pier Luigi Nimis and Régine Vignes Lebbe (eds.): “Tools for Identifying Biodiversity: Progress and Problems. Proceedings of the International Congress, Paris, September 20-22, 2010”, Trieste, EUT Edizioni Università di Trieste, 2010, pp. 307-313.
Abstract: There are serious economical reasons to pretend warranties in coffee species and varieties authenticity. Arabica adulteration with Robusta coffees, intentional or not, is carried out at different steps of the coffee chain, from plantation to beverage. We present a method based on a real-time PCR technique to perform: a) a qualitative analysis to evaluate the presence/ absence of a species in a sample; b) a quantitative analysis to amplify Robusta samples only, making possible the detection of less than 5% of Robusta in a mixture. Arabica cultivars sold as specialty coffees can be identified and certified by fingerprinting using SSR markers.
ISBN: 978-88-8303-295-0
Appears in Collections:Tools for Identifying Biodiversity: Progress and Problems

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