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Title: DNA Barcoding of Philippine plants
Authors: Agoo, Esperanza Maribel G.
Keywords: barcodingPhilippinesDNAidentificationflora
Issue Date: 2010
Publisher: EUT Edizioni Università di Trieste
Source: Esperanza Maribel G. Agoo, DNA Barcoding of Philippine plants, in Pier Luigi Nimis and Régine Vignes Lebbe (eds.): “Tools for Identifying Biodiversity: Progress and Problems. Proceedings of the International Congress, Paris, September 20-22, 2010”, Trieste, EUT Edizioni Università di Trieste, 2010, p. 341.
DNA barcoding is a technique that uses DNA sequence
data for species-level identification, for analyzing phylogenies and
interspecific variation and in population genetic studies. The DNA
barcodes determined by the Consortium of the Barcode of Life
(CBOL) to be the most effective in achieving these goals in plants
are the plastid genes namely trnH-psbA, rbcL, matK, accD, rpoB,
rpoc1, and trnL(UAA)-trnF(GAA). The goal of this barcoding project
is to test the genes, trnH-psbA, rbcL, and matK, in identification and
describing variation in some Philippine noteworthy indigenous plant
groups such as orchids, gingers, aroids, cinnamons, and cycads.
DNA are extracted and processed using standard protocol set by the
CBOL. The DNA are then kept in a cold storage facility in the DLSUCENSER
laboratory. Voucher specimens are also collected and are
now deposited in the DLSU-Manila Herbarium. Results of this study
show that these candidate barcodes can successfully discriminate
species including probable novelties of cycads, aroids, and
cinnamons. Quantitative analysis also suggest that rbcL and trnHpsbA
are very variable genes and can reflect greater interspecific
variation thus are the more useful barcodes in these plant groups.
Type: Book Chapter
ISBN: 978-88-8303-295-0
Appears in Collections:Tools for Identifying Biodiversity: Progress and Problems

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