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Title: Evolving biosensors: intelligent devices at the nanoscale
Authors: Marini, Monica
Supervisore/Tutore: Firrao, Giuseppe
Cosupervisore: Lazzarino, Marco
Issue Date: 29-Mar-2012
Publisher: Università degli studi di Trieste
In this work I present a novel approach to the use of biomolecules as constructive material for an autonomous DNA-based platform on which is possible to do sensing consequently actuating the object after the transduction of an environmental signal.
The new approach is based on a DNA origami obtained by folding a long polynucleotide with hundreds of shorter oligonucleotides and resulting in a well defined and ordered disks of a diameter of about 100nm. Each disk is composed of two main parts, an external ring and an internal disk, connected each other in only two diametrically opposite points. A linear single stranded DNA molecule, the probe, is inserted on the upper face of the internal moving disk, perpendicularly to the connections and to the axis of constrain; as far as the probe remains single stranded, the DNA-object appears planar, but when it gets in contact with its complementary ssDNA called “target”, forming a double stranded DNA, it opens the origami’s structure.
The realization of such autonomous organic structure is preliminary to its application in many contests. The actuation principle was first applied for the development of a revertable biosensing platform, where the addition of a third single stranded molecule, displaces the target from the probe restoring the initial state of the origami.
The same principle was also improved with real samples such as viral RNAs.
In this thesis, I report the setting up of the single components of the device: complex DNA based objects, the switching mechanism, the validation with real samples and the possible applications of the whole system.
Ciclo di dottorato: XXIV Ciclo
metadata.dc.subject.classification: SCUOLA DI DOTTORATO DI RICERCA IN NANOTECNOLOGIE
Keywords: DNA-Origami, actuation, autonomous, revertable
Type: Doctoral Thesis
Language: en
Settore scientifico-disciplinare: AGR/12 PATOLOGIA VEGETALE
NBN: urn:nbn:it:units-9169
Appears in Collections:Scienze fisiche

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