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Title: Development of electrochemical sensors by nanofabrication techniques for biological and medical diagnostics.
Authors: Virgilio, Francesca
Supervisore/Tutore: Tormen, Massimo
Cosupervisore: Ugo, Paolo
Issue Date: 27-Mar-2014
Publisher: Università degli studi di Trieste
In this work the fabrication and characterization of boron-doped diamond (BDD) nanoelectrode arrays are discussed. NEAs were obtained by creating an array of nanoholes by electron beam lithography (EBL) and Nanoimprint Lithography in a thin film of polycarbonate deposited on top of the macroelectrode. This approach leads to the formation of recessed nanoelectrodes. NEAs have been characterized with cyclic voltammetry and have provided voltammetric signals controlled by pure radial diffusion. Short ss-DNA have been immobilized on polycarbonate surface and hybridization experiments were carried out. Fluorescent and enzymatic labels were used in order to detect immobilization and hybridization of DNA. ECL preliminar experiment were also carried out on our NEAs.
Ciclo di dottorato: XXVI Ciclo
metadata.dc.subject.classification: SCUOLA DI DOTTORATO DI RICERCA IN NANOTECNOLOGIE
Keywords: Nanoelectrode Arrays
Boron-Doped Diamond
Electron Beam Lithography
Nanoimprint Lithography
Cyclic voltammetry
DNA detection
Language: en
Type: Doctoral Thesis
Settore scientifico-disciplinare: FIS/01 FISICA SPERIMENTALE
NBN: urn:nbn:it:units-12263
Appears in Collections:Scienze fisiche

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